Table 2 S

Primer and probe sequences for quantitative methylation analysis

 

gene                                                   sequence                                                                                                                               ref        

p16                         forward              5'-GGG GAG AGT AGA TAG CGG GC                           (1)

                                reverse               5'-AAC CAA TCA ACC GAA AAT TCC ATA

                                probe                  5'-FAM-TAC TCC CCG CCG CCG ACT CCA T-TAMRA

 

RASSF1A              forward              5'-GCG TTG AAG TCG GGG TTC                              (1)

                                Reverse              5'-CCC GTA CTT CGC TAA CTT TAA ACG

                                probe                  5'-FAM-ACA AAC GCG AAC CGA ACG AAA CCA-TAMRA

 

cyclinD2                forward              5'-TTT GAT TTA AGG ATG CGT TAG AGT ACG                  (1)

                                reverse               5'-ACT TTC TCC CTA AAA ACC GAC TAC G

                                probe                  5'-FAM-AAT CCG CCA ACA CGA TCG ACC CTA-TAMRA

 

DAP-K                   forward              5'-GGA TAG TCG GAT CGA GTT AAC GTC                      (1)

                                reverse               5'-CCC AAA CCC TAC CGC TAC G

                                probe                  5'-FAM-ATT ACC GAA TCC CCT CCG CGA AAA A-TAMRA

 

GSTp1                    forward              5'-GAT TTA GTA TTG GGG CGG AGC                          (2)

                                reverse               5'-CAC TAA TAA CGA AAA CTA CGA CGA CG

                                probe                  5'-FAM-CAA CGA AAA CCT CGC GAC CTC CG-TAMRA

 

HIN1                       forward              5'-GTT TTT TAG GGT TCG TCG GTC                          (2)

                                reverse               5'-TCC AAC GCC GAC CTC G

                                probe                  5'-FAM-TTA GGG TAC GGT CGT GAG CGG AGC-TAMRA

 

RIZ1                       forward              5'-GTC GTT CGA CGT TTT TTT CGA                          (2)

                                reverse               5'-CTA TTT CGC CGA CCC CG

                                probe                  5'-FAM-CGC CGC CGC CAT CTT AAC TCC-TAMRA

 

SOCS-1                  forward              5'-GGA TGG TAG TCG CGA GAG TTT C                        (2)

                                reverse               5'-ACG CGA CGC TAA CGC AAC G

                                probe                  5'-FAM-CCA ACA TAC GAC GCG ACG CCG-TAMRA

 

APC                        forward              5'-GAA CCA AAA CGC TCC CCA T                            (3)

                                reverse               5'-TTA TAT GTC GGT TAC GTG CGT TTA TAT

                                probe                  5'-FAM-CCC GTC GAA AAC CCG CCG ATT A-TAMRA

 

bAct                       forward              5'-TGG TGA TGG AGG AGG TTT AGT AAG T                    (4)

                                reverse               5'-AAC CAA TAA AAC CTA CTC CTC CCT TAA

                                probe                  5'-FAM-ACC ACC ACC CAA CAC ACA ATA ACA AAC ACA-TAMRA

 

 

(1) Lehmann et al., Am J Pathol, 160: 605-612., 2002

(2) this study

(3) Usadel et al., Cancer Res, 62: 371-375, 2002

(4) Eads et al., Cancer Res, 59: 2302-2306., 1999