The present project addresses the following aspects:
In our experiments we apply all current methods of molecular and cell biology as well as protein biochemistry and receptor pharmacology. In addition, we possess broad experiences in electrophysiology (patch-clamp), time-lapse microscopy and fluorescence microscopy. I our lab we also established the modern biophysical approaches for molecular imaging including, Förster resonance energy transfer (FRET) in cuvette and at single-cell level, fluorescence life-time measurements (FLIM) as well as bioluminescence resonance energy transfer (BRET). To analyse the expression level of different proteins we routinely use the quantitative RT-PCR analysis (TaqMan and SYBR Green) as well as single-cell RT-PCR techniques.