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Research

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    The present project addresses the following aspects:

    • Characterization of specific G-proteins and downstream effectors involved in GPCR-mediated signal transduction
    • Understanding of mechanisms providing the fine-tuning of multiple receptor-signalling pathways within the single cell and cellular networks. Analysis of cross-talk and feedback mechanisms
    • Subcellular localization of the 5-HT-mediated processes and the dynamic rearrangement of signalling domains in living cells. Dynamic distribution and clustering of defined 5-HT receptors in different cell types
    • Post-translational modifications of the signaling proteins and their role in the regulation of the signal transduction

     

    In our experiments we apply all current methods of molecular and cell biology as well as protein biochemistry and receptor pharmacology. In addition, we possess broad experiences in electrophysiology (patch-clamp), time-lapse microscopy and fluorescence microscopy. I our lab we also established the modern biophysical approaches for molecular imaging including, Förster resonance energy transfer (FRET) in cuvette and at single-cell level, fluorescence life-time measurements (FLIM) as well as bioluminescence resonance energy transfer (BRET). To analyse the expression level of different proteins we routinely use the quantitative RT-PCR analysis (TaqMan and SYBR Green) as well as single-cell RT-PCR techniques.