in Germany or Argentina
Lectures: Aug -Dec 2017
Lectures: Feb - May 2018
(Holidays: 1.6.18 -15.6.18)
Lectures: Jun 18 - Jan 19
(Holidays: 15.8.18 -5.10.18)
in Germany or Argentina
Soft Skills: Spanish for Germans
Molecular and Cellular Biochemistry Biology
Soft Skills: German for Argentinians
Thesis Workshop Scientific Writing 54 hours 2 ECTS
Lab Training I
Lab Training II
33 ECTS (Germans)
29 ECTS (Germans)
Contents (supported by MHH e-Learning platform):
1. Module: Soft Skills
Spanish for Germans. Students with Spanish knowledge pass test for credits.
2. Module: Basic Immunology
Development lymphoid organs. Innate immunity. Pathogen recognition. Antigen presentation. Adaptive immunity. Effector mechanisms. Signaling. Cytokines. Memory. Immune evasion. Immunodeficiencies. Allergy. Hypersensitivity. Autoimmunity. Diagnostics.
3. Module: Infection Biology
Medical Microbiology. Basic principles. Classification of bacteria. Morphology, cell wall structure and synthesis. Metabolism and growth. Genetics. Viruses, Fungi, Parasites: classification, structure, replication. Sterilization and disinfection. Microscopy. Molecular & Serological diagnostics. Pathogenic mechanisms. Antimicrobial agents. Acute and chronic diseases. Pathogenesis, dissemination, virulence, susceptibility. Prevention and control. Emerging diseases.
4. Module: Methods I
Sampling techniques: selection of research animals; inoculation/immunization techniques; recovery of samples. Culturing techniques, production of antibodies; Sample analysis: fluid sample analysis; cell phenotyping; cellular activation; cytokine/protein production and secretion; signaling; gene expression; tissue analysis; Practical training: isolation and stimulation of immune cells. ELISA. Western Blotting. Flow Cytometry.
5. Module: Molecular and Cellular Biology
Glycobiology of host-pathogen interactions. Theory: Role in cell recognition and host cell invasion. Glycan-based vaccines, diagnostics tools and glycoproteins as therapeutics. Signaling. Practical training: Sample preparation for glycan analysis. Extraction and purification. Fluorophore labelling, Glycan Cleanup. Sample preparation for HPLC.
6. Module: Advanced Immunology
Transplantation Immunology. Neuroimmunology. Mucosal Immunology. Intestinal Immune System, GALT. Tolerance versus effector responses. Microbiota. Functional food. Respiratory immune system. NALT and BALT. Immunobiology of the respiratory epithelium. Host-pathogen relationship. Natural antimicrobial peptides. Mucosal Vaccines. Practical training: Isolation of probiotic bacteria for functional food business. Evaluation of probiotic properties in vitro. Immunostaining and colocalization of proteins transiently expressed in cell lines.
7. Module: Lab Training I
Laboratory Training: new methodologies and theoretical concepts
8. Module: Soft Skills
German for Argentineans. See above.
9. Module: Translational Medicine
Vaccine development & Immunodiagnostics. Good Laboratory Practice. Ethical considerations. Clinical trials, Preclinical development: Immunogenicity and safety. Future vaccines. Trial design. Animal models, Adjuvants. Immunization route. Regulatory aspects. Entrepreneurial aspects. Safety issues and controversies.
Molecular Medicine. Virus bioengineering. Gene transfer strategies. Genetic therapy in immunodeficiencies and neurodegenerative diseases. iPS generation. Practical training: Experimental design of theoretical problems.
Molecular Diagnostics. Human genome. Genetic variation. New Generation Sequencing (NGS). Future technologies. Complete Genome Sequencing. Exome sequencing. Practical training: Experimental design of theoretical problems.
10. Module: Methods II
a. Multicolor Flow Cytometry
b. Detection and purification of specific cell types
c. Sample analysis, Sequencing
d. Fluorescence microscopy
e. Image processing
The students will be able to choose the courses according to their interests and the available vacancies for each course.
11. Module: Methods III
a. Animal models
b. Advanced methods for the analysis of the immune response Module: Thesis workshop
c. Laboratory Animal Handling. Laboratory health and safety. Ethical principles and laws. Alternative models to the use of animals.
12. Master Thesis including course Scientific writing (english)
Advanced research phase, independent research project. Master Thesis
as “tandem format”, including a Principal Investigator from each country (Germany and Argentina).