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Buildup of an automated -80°C repository at HUB



The central biobank of MHH, the Hannover Unified Biobank (HUB), established an automatic -80°C storage repository manufactured by Hamilton (BIOS). It is placed in the storeroom of the CRC Hannover. End of May 2015 the buildup of the BIOS device starts by the company. The connection of the BiOS system to the building services and the on-site nitrogen supply could already take place in July 2015. After an extensive test phase of Hamilton's facility, the system is handed over to the HUB in August, then HUB starts to do the Site Acceptance Test (SAT). By the winter of 2015 different tests, which are carried out by the HUB staff, run on the system and created and evaluated different temperature profiles for the individual processes. Just after a thorough examination in 2015 samples can be stored safely and monitored in the automated repository. This will be expected in winter 2015/2016. A large-scale storage of MHH and study samples in the BIOS system is expected in the spring of 2016th.


The structure of the automatic -80°C repository in the HUB is completed so far(updated 1/2016)


What is the advantage of an automated -80°C repository over conventional freezers and a manual import, export and stock transfer of the samples?


A secure, stable temperature-controlled and documented storage and retrieval of samples is important to a professional biobank. Manual samples on dry ice outsourcing pose a high risk that the nearby stored samples are impaired in their sample quality.

However, a prerequisite for the storage in the storage robot of the MHH biobank is the use of 2D bar-coded cryovials, as it will already be implemented through HUB for many MHH department and studies in externally funded projects since 2012. 2D barcode tubes allow for sample processing and storage as well as the compilation of sample shipments - the so-called picking - a secure and automated approach using laboratory and storage robots.

A manual compilation of sample shipments, in contrast to an automation solution, is time-consuming, error-prone, and caused by lack of samples compaction an unused dead volume in the sample storage, which is the negative impact on the profitability of a biobank. Economical to continually replenish vacant positions in the storage boxes and so make optimum use of the storage capacity of a biobank and to keep storage costs as low as possible is. In the HUB storage robot, these processes are automated and controlled temperature in the so-called pick station at a temperature of -80 ° C.

This also quality losses due to fluctuations in temperature in the rearrangement and the compilation of programs can be avoided. This form of storage is particularly suitable for short and medium-term storage. However, samples that are stored of quality and reliability reason in the gas phase of liquid nitrogen can be transported, without loss of quality, from the nitrogen tanks to the storage robot to do the corresponding work there. The BIOS is perfectly complemented by the existing aliquoting robot of body fluids (blood, urine, s.o.) and the automated DNA / RNA extraction system.

All devices are connected via interfaces to the common BIMS (Biobank Information Management System), so that a complete sample tracking of samples will be soon possible. Therefore, the CentraXX software from Kairos is established and integrated into the biobank processes in the HUB currently.