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Massenspektrometrie/Proteomics

Mass spectrometry laboratory

 

The MS lab is equipped with up to date instruments to perform MS-based investigations on single proteins and peptides and complete proteomes.

For more details on the MS-analysis click here (only in German).

 

Contact

 

Prof. Andreas Pich

Karin Agternkamp

Tel.: 0511-532-2808

Tel.: 0511-532-5614

E-mail: Pich.Andreasmh-hannover.de

 

 

Equipment

LTQ Orbitrap Velos (Thermo Fisher Scientific)

5800 MALDI TOF/TOF (ABSciex)

4000QTRAP (ABSciex)

Esquire 3000plus ESI-IT (Bruker Daltonik)

Nano HPLC (Dionex)

P/ACE capillary electrophoresis (Beckman Coulter)

Probot fraction collector (LC Packings, Dionex)

 

 

Collaborations and services

 

The MS lab is open for collaboration with other groups and provides services for protein identification, analysis of post translational modifications, and investigations of complete proteomes. Other projects are possible. We also offer consultations and advice for the experimental design of MS-experiments.

 

Instructions for protein identification

 

Samples to be analyzed:

  • proteins in acrylamide gel after 1-D or 2-D gel electrophoresis and staining with Coomassie, silver and other dyes
  • proteins in solution
  • proteins blotted on membranes (NC, PVDF)
  • proteins after immune precipitation
  • overexpressed proteins
  • purified proteins
  • mixtures of proteins
  • crude extracts for profiling experiments

 

Usually 0.1 to 1 pmol protein (5 to 50 ng of a 50 kDa protein) are sufficient for identification. Ideally, proteins have been separated in an acrylamide gel and were stained with Coomassie or silver. Please, fill out the sample form (PDF) and provide the complete gel or a picture of the gel where the bands of interest are indicated by numbers or names.

 

Guidelines for users (only in German)