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Methods

Biochemistry: Large scale production of recombinant proteins in E. coli; purification of proteins from  Dictyostelium cells and tissues; labeling of proteins with chromophors or fluorescent proteins; analyses of proteins by spectroscopic methods (pyrene-assays; fluorescence titrations); dynamic light scattering; functionalization of latex beads; TIRF-microscopy.

Molecular and Cell Biology: Generation of knock-outs and imaging of fluorescent proteins by confocal imaging; Differential interference, phase contrast and reflection interference contrast microscopy; Yeast-two-hybrid screens; Generation of antibodies.