SitemapImpressumDatenschutzerklärungdeutschenglish
MHH Logo

Prof. Dr. med. vet. Kirsten Haastert-Talini Research

Project: In vitro studies to characterize matrix qualities of simple and complex polySia-based materials
(DFG-FOR 548 for Prof. Dr. Claudia Grothe)

The biological effects of PolySia and PolySia-based materials on cultures of primary Neurons and peripheral glia (Schwann cells) ist evaluated in vitro. Evaluated Neurons are: sensory dorsal root ganglia neurons from neonatal rats, motoneurons from embryonic rat spinal cord or mesencephalic neural progenitor cells. Schwann cells are cultured from sciatic nerves of neonatal or adult rats but also from peripheral nerves of human donors. Cultures are analyzed with regard to cell adhesion, viability, proliferation rates as well as cell differentiation (neurite outgrowth) on the different PolySia-based substratum. 

 

 

 

 

 

 

 

 

 

 

 

 



 

Project: In vivo application of polySia-based materials in a rat model of peripheral nerve regeneration - morphological and functional regeneration (DFG-FOR 548 for Prof. Dr. Claudia Grothe)

Soluble PolySia and PolySia-based scaffold materials are evaluated in vivo. PolySia and PolySia-based materials are incorporated into nerve transplants which could be additionally substituted by transplanted Schwann cells. Exemplarily, the incorporation of PolySia-nanofibres and Schwann cells into tubular nerve transplants is depicted. Those biosynthetic nerve transplants are sutured between the stumps of transected sciatic nerves of adult rats to bridge a nerve gap. The outcome of the regeneration process is evaluated by functional and histomorphometrical methods. E.g., electrodiagnostic measurements are carried out in the anaesthetised animals by the end of the experiment to record compound muscle action potentials to determine the nerve conduction velocity. While histomorphometrical analysis of semi-thin cross sections through the regenerated tissue elucidates the number of regenerated myelinated axons. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Project:„Evaluation of the effects of a peptide fragment of the C3bot exoenzyme on peripheral nerve regeneration“

Förderpreis 2010 – Stiftung Sybille Assmus & NervClub

PD Dr. Kirsten Haastert-Talini

Aim: To study the effect of a 26 amino acid fragment of the clostridium botulinum C3-exoenzyme on peripheral nerve regeneration.


After sciatic nerve crush injury or nerve reconstruction by means of a nerve autotransplant, several solutions are injected into the lesion site or nerve sutures, respectively (control: buffer alone or nerve growth factor in buffer; test: C3-peptide in different concentrations or C3 wild type protein). The outcome of the regeneration process is evaluated by functional and histomorphometrical methods. Recovery of sensory and motor functions is measured on a regular basis in the awaken animals. Additionally, electrodiagnostic measurements (nerve conduction velocity) are carried out in the anaesthetised animals by the end of the experiments. Histomorphometrical analysis of semi-thin cross sections through the regenerated tissue elucidates the number of regenerated myelinated axons.

 

 

 

 

 back

 

 

 

 

 

 

 

 

 

 

Project: „Development of an „intelligent“ transplant for reconstruction of peripheral nerve gaps“ 

International Foundation Neurobionic

PD Dr. Kirsten Haastert-Talini

Aim: To develop a biomimetic nerve transplant which regeneration promoting effect equates that of nerve autotransplants.


To functionally restore long peripheral nerve gaps the regeneration processes have to be increased and steared. This could be achieved by electrical stimulaltion of the proximal nerve gap during reconstruction surgery and insertion of biodegradable sacffold maetrials to bridge the nerve gap. Regeneration-promoting factors/substances could be released from the scaffold materials, like e.g. nanofibers of polySialic acid or containments for seeding of therapeutical Schwann cells. Or transplanted genetically modified Schwann cells could enrich the nervegap with regeneration-promoting substances/factors in the manner of of ex vivo gene therapy. 
Different biohybrid nerve transplants will be sutured between the stumps of rat sciatic nerves to bridge long nerve gaps. Macroscopic regeneration will be evaluated by the appearance of gap bridging tissue reconnecting the nerve stumps at the end of the observation period. The outcome of the regeneration process is evaluated by functional and histomorphometrical methods. Electrodiagnostic measurements (nerve conduction velocity) are carried out in the anaesthetised animals by the end of the experiments to evaluate nerve function. Histomorphometrical analysis of semi-thin cross sections through the regenerated tissue elucidates the number of regenerated myelinated axons.

 

 

 

 

 back