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Supplementary Material

Supplementary Information

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Suppl. Fig. 1: Blocking of CD20 expression in human B and T cells. Human PBMC were preincubated with increasing amounts of unlabeled CD20 antibodies or IgG (as control) for 30 min. After washing, cells were stained with CD20PE and CD3PerCP antibodies. A: 20µl IgG control; amount of blocking CD20 antibodies: B= 1µl, C= 10µl, D= 20µl.

 

 

CD19 and CD20 mRNA expression in human T and B cells. Human PBMC were sorted into 3 populations (CD3+CD20+, CD3+CD20- and CD3-CD20+) with a FACSAria cell sorter. CD19 and CD20 specific mRNA expression was analyzed by quantitative real time PCR using an iCycler (Biorad, Munich, Germany).

 

 

 

 

Suppl. Fig. 3a: Receptor repertoires of a/b T cell populations. PBMC were gated on CD3+CD20+ and CD3+CD20- cells and the usage of Vb chains were determined by multicolour flow cytometry. Percentages of positive T cells for the different Vb chains (as indicated on the x-axis) are depicted for CD20+ (white bars) and CD20- T cells (black bars), which were analyzed from peripheral blood of five healthy individuals.

 

 

 

 

Suppl. Fig. 3b: Receptor repertoires of a/b T cell populations. PBMC were gated on CD3+CD20+ and CD3+CD20- cells and the usage of Vb chains were determined by multicolour flow cytometry. Percentages of positive T cells for the different Vb chains (as indicated on the x-axis) are depicted for CD20+ (white bars) and CD20- T cells (black bars), which were analyzed from peripheral blood of eight RA patients.

 

 

 

 

Suppl. Fig 4: Proliferative capacity of CD20+ and CD20- T lymphocytes. PBMC were sorted into CD3+CD20+ (white bar) and CD3+CD20- (black bar) T cells. Cells were activated by CD3 cross-linking for 72h and H3-thymidine was added 24h before harvesting. Incorporation of the H3-nuclides is given in counts per minute (cpm).

 

 

suppl. Table 1