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Project U

Project U

 

 

Name:

Prof. Dr. Thomas Werfel

 

Institution:

Department of Dermatology and Allergology

 

Telephone:

0049-511-9246 276, 0049-511-9246 0

 

Email:

Thomas_Werfelt-online.de

 

Research focus:

Investigation of the role of T-cells and antigen presenting cells in the pathogenesis of chronic inflammatory skin diseases, in particular atopic dermatitis and contact dermatitis

 

available project:

Project: Characterization of human dendritic cells in atopic dermatitis and contact dermatitis, in particular with regard to expression of anaphylatoxin receptors and responsiveness to anaphylatoxins C3a und C5a

 

Group members:

Prof. Dr. Thomas Werfel, Principal Investigator Dr. Ralf Gutzmer, Postdoctoral Fellow Dr. Miriam Wittmer, Postdoctoral Fellow Dr. Kristine Breuer, Postdoctoral Fellow Susanne Mommert, Biologist Brigitta Köther, Biologist Tanja Stünkel, Technician Viola Kohlrautz, Technician

 

Methods:

Cell culture, flowcytometry, immunomagnetic cell separation, preparation of T cell lines and clones from peripheral blood and skin, real time LightCycler PCR, proliferation assays, ELISA, Western Blot

 

Key References:

Gutzmer R, Langer K, Lisewski M, Mommert S, Kapp A, Werfel T: Expression and function of histamine receptors 1 and 2 on human monocyte derived dendritic cells. J. Allergy Clin. Immunol, in press

 

Gutzmer R, Mommert S, Kiehl P, Wittmann M, Kapp A, Werfel T: Detection of clonal T-cell receptor ? gene rearrangements in cutaneous T-cell lymphoma by LightCycler-PCR. J Invest Dermatol 116: 926-932 (2001)

 

Kirchhoff K, Weinmann O, Zwirner J, Begemann G, Götze O, Kapp A, Werfel T: Detection of anaphyatoxin receptors on CD 83+ dendritic cells derived from human skin. Immunology 103:210-7 (2001)

 

Werfel T, Morita A, Grewe M, Renz H, Wahn U, Krutmann J, Kapp A: Allergen-specificity of skin infiltrating T cells is not restricted to a type 2 cytokine pattern in chronic lesions of atopic dermatitis. J Invest Dermatol 107: 871-6 (1996)

 

Werfel T, Zwirner J, Oppermann M, Sieber A, Begemann G, Drommer W, Kapp A, Götze O: CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin. J Immunol 157: 1729-1735 (1996)

 

Werfel T, Hentschel M, Kapp A, Renz H: Dichotomy of blood- and skin-derived IL-4 producing allergen-specific T-cells and restricted V beta repertoire in nickel-mediated contact dermatitis. J Immunol 158: 2500-5 (1997)

 

Werfel T, Kirchhoff K, Wittmann M, Begemann G, Kapp A, Heidenreich F, Götze O, Zwirner J: Activated human T lymphocytes express a functional C3a receptor. J Immunol 165: 6599-6605 (2000)

 

Wittmann M, Zwirner J, Larsson V-A, Kirchhoff K, Begemann G, Kapp A, Götze O, Werfel T: C5a suppresses the production of IL-12 by IFN? primed and LPS challenged human monocytes. J Immunol 162: 6763-6769 (1999)

 

Wittmann M, Larsson VA, Schmidt P, Begemann G, Kapp A, Werfel T: Suppression of interleukin-12 production by human monocytes after preincubation with lipopolysaccharide. Blood 94: 1717 (1999)

 

Wittmann M, Kienlin P, Mommert S, Kapp A, Werfel T: Suppression of IL-12 production by sCD40L-evidence for involvement of the p44/42 MAPK pathway. J Immunol. in press

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