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Project L

Project L




Dr. Frauke Mattner, Dr. Axel Kola, Dr.Petra Gastmeier



Medical Mikrobiology and Hygiene



0049-511-532 8675




Research focus:

The prevalence of multi-resistant bacteria increased markedly in recent years. New problems arised from extended-spectrum ß-lactamase (ESBL)-producing Klebsiella pneumoniae and E. coli in cause of following problems:

  1. The mechanism of resistance is due to plasmids, which can be exchanged between different species. Many outbreaks due to transmission have been reported.

  2. The initial antimicrobial susceptibility testing can show sensible results in vitro, whereas in vivo the mechanism of resistance will be activated and the antibiotic therapy will fail.

  3. The laboratory diagnostic is established using special disk diffusion method, which are often difficult to interpret.

  4. According to the literature a multitude of genetic variants coding for the ESBLs exists. To facitilitate and specify the diagnostic of ESBL-producing bacteria, a PCR method for resistance genes could prove usefull. Real-time PCR and melting curve analysis should be used to detect SHV, TEM and AmpC-ß-lactamase genes.

  5. Reliable diagnostic possibilities are required to perform a prospective surveillance of infections with ESBL-producing strains. This surveillance would provide valid clinical data to the epidemiology of ESBL-producing bacteria. Molecular typing (PFGE, RAPD) methods would allow to distinguish between transmission and emergence of ESBLs.



  1. To establish real-time PCR-based diagnostic of ESBLs

  2. To investigate transmission of ESBL producing bacteria by PFGE, RAPD


Group members:

Dr. Frauke Mattner
Dr. Axel Kola
Dr. Iris Chaberny
Prof. Dr. Petra Gastmeier



Real-Time PCR (Light cycler), PFGE, RAPD


Key References:

Randegger, C. et al. Real-Time PCR and Melting Point Analyses for Reliable and Rapid Detection of SHV Extended-Spectrum ß-Lactamases. Antimicrobial Agents and Chemotherapy 2001: 1730-6


Cockerill, F. Minirewiew: Genetic Methods for Assessing Antimicrobial Resistance. Antimicrobial Agents and Chemotherapy 1999: 199-212


Lautenbach, E. et al. Extended-Spectrum ß-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae: Risk Factors for Infection and Impact of Resistance on Outcomes. CID 2001:32:1162-1171


Bradford, P.A. Extended-Spectrum ß-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clinical Microbiology Reviews, 2001:933-951


Gastmeier P, Sohr D, Just HM, Nassauer A, Daschner F, Ruden H.How to survey nosocomial infections. Infect Control Hosp Epidemiol 2000 Jun;21(6):366-70

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