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Project F

Project F

 

 

Name:

Prof. Dr. med. Hermann Haller

 

Institution:

Department of Nephrology

 

Telephone:

0049-511-532 6319

 

Email:

haller.hermannmh-hannover.de,

nephrologiemh-hannover.de

 

Research focus:

Mechanisms of Signal Transduction - Role of Lipids Rafts We have recently generated a Knock-out-Mouse for Caveolin-1 and -2. These mice lack caveolae inall cell types of the body. They are born without any problems and display a different interesting phenotypes. We now want to analyze the role of caveolei in the kidney. From our preliminary observations these mice develop focal sclerosis and proteinuria. The hypothesis is that caveolae are important for gene expression in podocytes and endothelial cells. We want to analyze the underlying signal transduction and test the hypothesis that the wnt-signalling pathway is affected by the lack of caveolae.

 

Projects:

Slit membranes and proteinuria

Blood brain barrier in hypertension

Signal transduction and cell migration

 

Methods:

In vivo: Immunohistochemistry, electromicroscopy, in-situ hybridisation

In vitro: Confocal microscopy, immunoprecipitation, differential display, micro array techniques

 

Group members:

Faikah Abou-Rebyeh

Rong Song

Carsten Lindschau

Jan Menne

Torsten Kirsch

Inna Dumler

Harald Mischak

Andrea Lippoldt

 

Key References:

  1. Drab M, Verkade P, Elger M, Kasper M, Lohn M, Lauterbach B, Menne J, Lindschau C, Mende F, Luft FC, Schedl A, Haller H, Kurzchalia TV: Loss of caveolae, vascular dysfunction and pulmonary defects in caveolin-1 gene-disrupted mice. Science. 2001 28;293 (5539): 2449-52

  2. Lippoldt A, Kniesel U, Liebner S, Kalbacher H, Kirsch T, Wolburg H, Haller H: Structural alterations of tight junctions are associated with loss of polarity in stroke-prone spontaneously hypertensive rat blood-brain barrier endothelial cells. Brain Res. 2000; 885(2):251-261

  3. Lohn M, Furstenau M, Sagach V, Elger M, Schulze W, Luft FC, Haller H, Gollasch M. Ignition of calcium sparks in arterial and cardiac muscle through caveolai. Circ Res. 2000; 87 (11): 1043-9

  4. Maasch C, Wagner S, Lindschau C, Alexander G, Buchener K, Gollasch M, Luft FC, Haller, H. Protein kinase Calpha targeting is regulated by temporal and spatial changes in intracellular free calcium concentration [Ca (2+)] (i). FASEB J 2000; 14 (11): 1653-1663

  5. Kirsch T, Wellner M, Luft FC, Haller H, Lippoldt A. Altered gene expression in cerebral capillaries of stroke-prone spontaneously hypertensive rats. Brain Res. 2001, Aug 10; 910(1-2):106-15

  6. Stepanova V, Jerke U, Sagach V, Lindschau C, Dietz R, Haller H, Dumler I. Urokinase-dependent human vascular smooth muscle cell adhesion requires selective vitronectin phosphorylation by ecto-protein kinase CK2. J Biol Chem. 2001

 

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