Publications

Browning, C., Shneider, M., Bowman, V., Schwarzer, D., and Leiman, P. (2012) Phage Pierces the Host Cell Membrane With the Iron-Loaded Spike. Structure 20(2):326-39.

Schaper, W.,Bentrop, J., Ustinova, J., Blume, L., Kats, E., Tiralongo, J., Weinhold, B., Bastmeyer, M., and Münster-Kühnel, A. (2012) Identification and biochemical characterization of two functional CMP-sialic acid synthetases in Danio rerio. J. Biol. Chem. 2012 Feb 20. [Epub ahead of print]

Sethi, M.K., Buettner, F.F., Ashikov, A., Krylov, V.B., Takeuchi, H., Nifantiev, N.E., Haltiwanger, R.S., Gerardy-Schahn, R., and Bakker, H. (2012) Molecular Cloning of a Xylosyltransferase That Transfers the Second Xylose to O-Glucosylated Epidermal Growth Factor Repeats of Notch. J. Biol. Chem. 287(4):2739-48.

Vogt J., Glumm R., Schlüter L., Schmitz D., Rost B.R., Streu N., Rister B., Bharathi B.S., Gaggiannis D., Hildebrandt H., Weinhold B., Mühlenhoff M., Naumann T., Savaskan N.E., Brauer A.U., Reutter W., Heimrich B., Nitsch R, Horstkorte R. (2012) Homeostatic regulation of NCAM-polysialylation is critical for correct synaptic targeting. Cell. Mol. Life Sci. 69 (7): 1179-91.

Hildebrandt H., Mühlenhoff M., Gerardy-Schahn, R. (2012) Polysialinsäure – ein Zucker reguliert die Hirnentwicklung. BioSpektrum 18 (1): 26-29, Review (German).

Bolborea M., Laran-Chich M.P., Rasri K., Hildebrandt H., Govitrapong P., Simonneaux V., Pevet P., Steinlechner S., Klosen P. (2011). Melatonin controls photoperiodic changes in tanycyte vimentin and neural cell adhesion molecule expression in the Djungarian hamster (Phodopus sungorus). Endocrinology 152 (10):3871-3883.

Chen, R., John, J., Rode, B., Hitzmann, B., Gerardy-Schahn, R., Kasper, C., and Scheper, T. (2011) Comparison of Polysialic Acid Production in Escherichia Coli K1 During Batch Cultivation and Fed-Batch Cultivation Applying Two Different Control Strategies. J. Biotechnol. 154(4):222-9.

Dickmanns, A., Damerow, S., Neumann, P., Schulz, E.C., Lamerz, A.C., Routier, F.H., and Ficner, R. (2011) Structural Basis for the Broad Substrate Range of the UDP-Sugar Pyrophosphorylase From Leishmania Major. J. Mol. Biol. 405(2):461-78.

Eggers K., Werneburg S., Schertzinger A., Abeln M., Schiff M., Scharenberg M.A., Burkhardt H., Mühlenhoff M., Hildebrandt H. (2011) Polysialic acid controls NCAM signals at cell-cell contacts to regulate focal adhesion independent from FGF receptor activity. J. Cell Sci. 124 (19):3279-3291.

Hu, H., Eggers, K., Chen, W., Garshasbi, M., Motazacker, M.M., Wrogemann, K., Kahrizi, K., Tzschach, A., Hosseini, M., Bahman, I., Hucho, T., Mühlenhoff, M., Gerardy-Schahn, R., Najmabadi, H., Ropers, H.H., and Kuss, A.W. (2011) ST3GAL3 Mutations Impair the Development of Higher Cognitive Functions. Am. J. Hum. Genet. 89(3):407-14.

Kraft, B., Johswich, A., Kauczor, G., Scharenberg, M., Gerardy-Schahn, R., and Bakker, H. (2011) "Add-on" Domains of Drosophila Beta1,4-N-Acetylgalactosaminyltransferase B in the Stem Region and Its Pilot Protein. Cell Mol. Life Sci. 68(24):4091-100.

Rey-Gallardo, A., Delgado-Martin, C., Gerardy-Schahn, R., Rodriguez-Fernandez, J.L., and Vega, M.A. (2011) Polysialic Acid Is Required for Neuropilin-2a/b-Mediated Control of CCL21-Driven Chemotaxis of Mature Dendritic Cells and for Their Migration in Vivo. Glycobiology 21(5):655-62.

Schiff M., Röckle I., Burkhardt H., Weinhold B., Hildebrandt H. (2011) Thalamocortical pathfinding defects precede degeneration of the reticular thalamic nucleus in polysialic acid-deficient mice. J. Neurosci. 31 (4): 1302-1312.

Schulz, E.C., Bergfeld, A.K., Ficner, R., and Mühlenhoff, M. (2011) Crystal Structure Analysis of the Polysialic Acid Specific O-Acetyltransferase NeuO. PLoS. One. 6(3):e17403.

Tefsen, B., Ram, A.F., van, D., I, and Routier, F.H. (2011) Galactofuranose in Eukaryotes: Aspects of Biosynthesis and Functional Impact. Glycobiology [Epub ahead of print]

Valentiner U., Mühlenhoff M., Lehmann U., Hildebrandt H., Schumacher U. (2011) Expression of the neural cell adhesion molecule and polysialic acid in human neuroblastoma cell lines. Int. J. Oncol. 39 (2): 417-424.

Böhm, R., Freiberger, F., Stummeyer, K., Gerardy-Schahn, R., von Itzstein, M., and Haselhorst, T. (2010) Neisseria meningitidis serogroup B polysialyltransferase: insights into substrate binding. Chembiochem. 11(2):170–4.

Chaipan, C., Steffen, I., Tsegaye, T.S., Bertram, S., Glowacka, I., Kato, Y., Schmökel, J., Münch, J., Simmons, G., Gerardy-Schahn, R., and Pöhlmann, S. (2010) Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2. Retrovirology. 7:47.

Damerow, S., Lamerz, A.-C., Haselhorst, T., Führing, J., Zarnovican, P., von Itzstein, M., and Routier, F. (2010) Leishmania UDP-sugar pyrophosphorylase: the missing link in galactose salvage? J. Biol. Chem. 285(2):878–87.

Dichtl, K., Ebel, F., Dirr, F., Routier, F.H., Heesemann, J., and Wagener, J. (2010) Farnesol misplaces tip-localized Rho proteins and inhibits cell wall integrity signalling in Aspergillus fumigatus. Mol. Microbiol. 76(5):1191–204.

Galuska, S.P., Geyer, H., Bleckmann, C., Röhrich, R.C., Maass, K., Bergfeld, A.K., Mühlenhoff, M., and Geyer, R. (2010) Mass spectrometric fragmentation analysis of oligosialic and polysialic acids. Anal. Chem. 82(5):2059–66.

Galuska, S.P., Geyer, H., Weinhold, B., Kontou, M., Rohrich, R.C., Bernard, U., Gerardy-Schahn, R. , Reutter, W., Münster-Kühnel, A., and Geyer, R. (2010) Quantification of nucleotide-activated sialic acids by a combination of reduction and fluorescent labeling. Anal. Chem. 82(11):4591–8.

Galuska, S.P.*, Rollenhagen, M.*, Kaup, M., Eggers, K., Oltmann-Norden, I., Schiff, M., Hartmann, M., Weinhold, B., Hildebrandt, H., Geyer, R., Mühlenhoff, M.*, and Geyer, H.* (2010) Synaptic cell adhesion molecule SynCAM 1 is a target for polysialylation in postnatal mouse brain. Proc. Natl. Acad. Sci. USA. 107(22):10250-5.  

Haastert-Talini, K., Schaper-Rinkel, J., Schmitte, R., Bastian, R., Mühlenhoff, M., Schwarzer, D., Draeger, G., Su, Y., Scheper, T., Gerardy-Schahn, R., and Grothe, C. (2010) In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants. Tissue Eng. Part A. 16(10):3085–98.

Hildebrandt, H., Mühlenhoff, M., and Gerardy-Schahn, R. (2010) Polysialylation of NCAM. Adv. Exp. Med. Biol. 663:95–109, Review.

Kochlamazashvili, G., Senkov, O., Grebenyuk, S., Robinson, C., Xiao, M.F., Stummeyer, K., Gerardy-Schahn, R., Engel, A.K., Feig, L., Semyanov, A., Suppiramaniam, V., Schachner, M., and Dityatev, A. (2010) Neural cell adhesion molecule-associated polysialic acid regulates synaptic plasticity and learning by restraining the signaling through GluN2B-containing NMDA receptors. J. Neurosci. 30(11):4171–83.

Kotz, A., Wagener, J., Engel, J., Routier, F., Echtenacher, B., Pich, A., Rohde, M., Hoffmann, P., Heesemann, J., and Ebel, F. (2010) The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity. Fungal Genet. Biol. 47(2):169–78.

Koutsoudaki, P.N. , Hildebrandt, H., Gudi, V., Skripuletz, T., Skuljec, J., and Stangel, M. (2010) Remyelination is accelerated in St8siaIV deficient mice after cuprizone induced demyelination. Neuroscience 171(1):235–44.

Kustermann, S., Hildebrandt, H., Bolz, S., Dengler, K. and Kohler, K. (2010) Genesis of rods in the zebrafish retina occurs in a microenvironment provided by polysialic acid-expressing Müller glia.  J. Comp. Neurol. 518(5):636–46.

Lamerz, A.C., Damerow, S., Kleczka, B., Wiese, M., van Zandbergen, G., Lamerz, J., Wenzel, A., Hsu, F.F., Turk, J., Beverley, S.M., and Routier, F.H. (2010) Deletion of UDP-Glucose Pyrophosphorylase Reveals a UDP-Glucose Independent UDP-Galactose Salvage Pathway in Leishmania major. Glycobiology. 20(7):872-82.

Nacher, J., Guirado, R., Varea, E., Alonso-Llosa, G., Röckle, I., and Hildebrandt, H. (2010) Divergent impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid (PSA) expression in immature neurons and interneurons of the adult cerebral cortex. Neuroscience 167(3):825–37.

Pekcec, A., Weinhold, B., Gerardy-Schahn, R., and Potschka, H. (2010) Polysialic acid affects pathophysiological consequences of status epilepticus. Neuroreport. 21(8):549-53.

Rey-Gallardo, A., Escribano, C., Delgado-Martín, C., Rodriguez-Fernández, J.L., Gerardy-Schahn, R., Rutishauser, U., Corbi, A.L., and Vega, M.A. (2010) Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21 Glycobiology. 20(9):1139-46. 

Schulz, E.C., Dickmanns, A., Urlaub, H., Schmitt, A., Mühlenhoff, M., Stummeyer, K., Schwarzer, D., Gerardy-Schahn, R., and Ficner, R. (2010) Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding. Nat. Struct. Mol. Biol. 17(2):210–5.

Schulz, E.C., Neumann, P., Gerardy-Schahn, R., Sheldrick, G.M., and Ficner, R. (2010) Structure analysis of endosialidase NF at 0.98 Å resolution. Acta Crystallogr. D Biol. Crystallogr. 66(Pt 2):176–80.

Schulz, E.C., Schwarzer, D., Frank, M., Stummeyer, K., Mühlenhoff, M., Dickmanns, A., Gerardy-Schahn, R., and Ficner, R. (2010) Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein EndoNF. J. Mol. Biol. 397(1):341–51.

Sethi, M.K., Büttner, F.F., Krylov, V.B., Takeuchi, H., Nifantiev, N.E., Haltiwanger, R.S., Gerardy-Schahn, R., and Bakker, H. (2010) Identification of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats. J. Biol. Chem. 285(3):1582–6.

Bakker, H., Oka, T., Ashikov, A., Yadav, A., Berger, M., Rana, N.A., Bai, X., Jigami, Y., Haltiwanger, R.S., Esko, J.D., and Gerardy-Schahn, R. (2009) Functional UDP-xylose transport across the ER/Golgi membrane in a Chinese hamster ovary cell mutant defective in UDP-xylose synthase. J. Biol. Chem. 284(4):2576–83.

Bergfeld, A. K., Claus, H., Lorenzen, N. K., Spielmann, F., Vogel, U., and Mühlenhof, M. (2009) The polysialic acid specific O-acetyltransferase OatC from Neisseria meningitidis serogroup C evolved apart from other bacterial sialate:O-acetyltransferases. J. Biol. Chem. 284(1):6–16.

Boztug, K., Appaswamy, G.*, Ashikov, A.*, Schäffer, A.A., Salzer, U., Diestelhorst, J., Germeshausen, M., Brandes, G., Lee-Gossler, J., Noyan, F., Gatzke, A.K., Minkov, M., Greil, J., Kratz, C., Petropoulou, T., Pellier, I., Bellanné-Chantelot, C., Rezaei, N., Mönkemöller, K., Irani-Hakimeh, N., Bakker, H., Gerardy-Schahn, R., Zeidler, C., Grimbacher, B., Welte, K., and Klein, C. (2009) A syndrome with congenital neutropenia and mutations in G6PC3. N. Engl. J. Med. 360(1):32–43.

Claus, H.*, Stummeyer, K.*, Batzilla, J., Mühlenhoff, M. and Vogel, U. (2009) Amino acid 310 determines the donor substrate specificity of serogroup W-135 and Y capsule polymerases of Neisseria meningitidis. Mol. Micro. 71(4):960–71.

Drake, P. M, Stock, C. M, Nathan, J. K, Gip, P., Golden, K. P., Weinhold, B., Gerardy-Schahn, R., and Bertozzi, C.R. (2009) Polysialic acid governs T-cell development by regulating progenitor access to the thymus. Proc. Natl. Acad. Sci. U S A. 106(29):11995–2000.

Engel, J., Schmalhorst, P., Dörk-Bousset, T., Ferrières, V., and Routier , F. (2009) A Single UDP-galactofuranose Transporter Is Required for Galactofuranosylation in Aspergillus fumigatus. J. Biol. Chem.284(49):33859–68.

Hese, K., Otto, C., Routier, F.H., and Lehle, L. (2009) The yeast oligosaccharyltransferase complex can be replaced by STT3 from Leishmania major. Glycobiology. 19(2):160–71.

Hildebrandt, H., Mühlenhoff, M., Oltmann-Norden, I., Röckle, I., Burkhardt, H., Weinhold, B., and  Gerardy-Schahn, R. (2009) Imbalance of neural cell adhesion molecule and polysialyltransferase alleles causes defective brain connectivity. Brain. 132(Pt 10):2831–8.

Johswich, A., Kraft, B., Wuhrer, M., Berger, M., Deelder, A.M., Hokke, C.H., Gerardy-Schahn, R., and Bakker, H. (2009) Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot. J. Cell Biol. 184(1):173–83.

Jungnickel, J., Brämer, C., Bronzlik, P., Lipokatic-Takacs, E., Weinhold, B., Gerardy-Schahn, R., and Grothe, C. (2009) Level and localization of polysialic acid is critical for early peripheral nerve regeneration. Mol. Cell Neurosci. 40(3):374–81.

Klehr, M. , Koehl, U., Mühlenhoff, M., Tawadros, S., Fischer, Schomäcker T.K., Heuckmann, J.M., Bochennek, K., and Jensen, M. (2009) The Novel Chimeric Anti-NCAM (Neural Cell Adhesion Molecule) Antibody ch.MK1 Displays Antitumor Activity in SCID Mice but Does not Activate Complement-Dependent Cytolysis (CDC). J. Immunother. 32(5):442–451.

Koutsoudaki, P.N., Skripuletz, T., Gudi, V., Moharregh-Khiabani, D., Hildebrandt, H., Trebst, C., and Stangel, M. (2009) Demyelination of the hippocampus is prominent in the cuprizone model. Neurosci. Lett. 451(1):83–8.

Mordhorst, I. L., Claus, H., Ewers, C., Lappann, M., Schoen, C., Elias, J., Batzilla, J., Dobrindt, U., Wieler, L. H., Bergfeld, A. K., Mühlenhoff, M., and Vogel, U. (2009). O-acetyltransferase gene neuO is segregated according to phylogenetic background and contributes to environmental desiccation resistance in Escherichia coli K1.,  Environ. Microbiol. 11(12):3154–65.

Mühlenhoff, M., Oltmann-Norden, I., Weinhold, B., Hildebrandt, H., and Gerardy-Schahn, R. (2009) Brain development needs sugar: the role of polysialic acid in controlling NCAM functions. Biol Chem. 2009 Jul; 390(7):567–74, Review.

Oschlies, M.; Dickmanns, A.; Haselhorst, T.; Schaper, W.; Stummeyer, K.; Tiralongo, J.; Weinhold, B.; Gerardy-Schahn, R.; von Itzstein, M.; Ficner, R. ; Münster-Kühnel, A.-K., (2009) A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases  provides a tetramerization interface for the physiologically active enzyme. J. Mol. Biol. 393(1):83–97.

Schiff, M., Weinhold, B., Grothe, C., and Hildebrandt, H. (2009) NCAM and polysialyltransferase profiles match dopaminergic marker gene expression but polysialic acid is dispensable for development of the midbrain dopamine system.  J. Neurochem. 110: 1661–1673.

Schwarzer, D., Stummeyer, K., Haselhorst, T., Freiberger, F., Rode, B., Grove, M., Scheper, T., von Itzstein, M., Mühlenhoff, M., and Gerardy-Schahn, R. (2009) Proteolytic release of the intramolecular chaperone domain confers processivity to endosialidase F. J. Biol. Chem. 284(14):9465–74.

Bakker, H., Routier, F., Ashikov, A., Neumann, D., Bosch, D., and Gerardy-Schahn, R. (2008) A CMP-sialic acid transporter cloned from Arabidopsis thaliana. Carbohydr Res. 343(12):2148-52.

Berski, S. , van Bergeijk, J., Schwarzer, D., Stark, Y., Kasper, C., Scheper, T., Grothe, C., Gerardy-Schahn, R., Kirschning, A., and Dräger, G. (2008) Synthesis and Biological Evaluation of a Polysialic Acid Based Hydrogel as Enzymatically Degradable Scaffold Material for Tissue Engineering. Biomacromolecules. 9(9):2353-9.

Caffaro, C.E., Luhn, K., Bakker, H., Vestweber, D., Samuelson, J., Berninsone, P., and Hirschberg, C.B. (2008) A Single Caenorhabditis elegans Golgi Apparatus-Type Transporter of UDP-Glucose, UDP-Galactose, UDP-N-Acetylglucosamine, and UDP-N-Acetylgalactosamine. Biochemistry 47(14):4337-4344.

Drake, P.M., Nathan. J.K., Stock, C.M., Chang, P.V., Muench, M.O., Nakata, D., Reader, J.R., Gip, P., Golden, K.P., Weinhold, B., Gerardy-Schahn, R., Troy, F.A. 2nd, and Bertozzi, C.R. (2008) Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. J Immunol. 181(10):6850-8.

Galuska, S.P., Geyer, R., Gerardy-Schahn, R., Mühlenhoff, M., and Geyer, H. (2008) Enzyme-dependent variations in the polysialylation of the neural cell adhesion molecule NCAM in vivo. J Biol Chem. 283(1):17-28.

Haile, Y., Berski, S., Dräger, G., Nobre, A., Stummeyer, K., Gerardy-Schahn, R., and Grothe, C. (2008) The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells. Biomaterials. 29(12):1880-91.

Hartlieb, S., Günzel, A., Gerardy-Schahn, R., Münster-Kühnel, A.K., Kirschning, A., and Dräger, G. (2008) Chemoenzymatic synthesis of CMP-N-acetyl-7-fluoro-7-deoxy-neuraminic acid. Carbohydr Res. 343(12):2075-82.

Hildebrandt, H., Mühlenhoff, M., and Gerardy-Schahn, R. (2008) Polysialylation of NCAM. Neurochem. Res. 2008 May 7 (in press).

Oltmann-Norden, I., Galuska, S.P., Hildebrandt, H., Geyer, R., Gerardy-Schahn, R., Geyer, H., and Mühlenhoff, M. (2008) Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J Biol Chem. 283(3):1463-71. 

Pekcec, A., Fuest, C., Mühlenhoff, M., Gerardy-Schahn, R., and Potschka, H. (2008) Targeting epileptogenesis-associated induction of neurogenesis by enzymatic depolysialylation of NCAM counteracts spatial learning dysfunction but fails to impact epilepsy development. J Neurochem. 105(2):389-400.

Potschka, H., Pekcec, A., Weinhold, B., and Gerardy-Schahn, R. (2008) Deficiency of neural cell adhesion molecule or its polysialylation modulates pharmacological effects of the AMPA receptor antagonist NBQX. Neuroscience 152(4):1093-8.

Rode, B., Endres, C., Ran, C., Stahl, F., Beutel, S., Kasper, C., Galuska, S., Geyer, R., Mühlenhoff, M., Gerardy-Schahn, R., and Scheper, T. (2008) Large scale production and homogenous purification of long chain polysialic acids from E. coli K1. J Biotech. 135(2):202-9.

Röckle, I., Seidenfaden, R., Weinhold, B., Mühlenhoff, M., Gerardy-Schahn, R., and Hildebrandt, H. (2008) Polysialic acid controls NCAM-induced differentiation of neuronal precursors into calretinin-positive olfactory bulb interneurons. Dev Neurobiol. 68(9):1170-1184.

Schmalhorst, P.S., Krappmann, S., Vervecken, W., Rohde, M., Müller, M., Braus, G.H., Contreras, R., Braun, A., Bakker, H., and Routier, F.H. (2008) Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus Fumigatus. Eukaryot Cell. 7(8):1268-77.

Schreiber, S.C., Giehl, K., Kastilan, C., Hasel, C., Mühlenhoff, M., Adler, G., Wedlich, D., and Menke, A. (2008) Polysialylated NCAM Represses E-Cadherin-Mediated Cell-Cell Adhesion in Pancreatic Tumor Cells. Gastroenterology. 134(5):1555-66.

Stolz, A., Haines, N., Pich, A., Irvine, K.D., Hokke, C.H., Deelder, A.M., Gerardy-Schahn, R., Wuhrer, M., and Bakker, H. (2008) Distinct contributions of beta4GalNAcTA and beta4GalNAcTB to Drosophila glycosphingolipid biosynthesis. Glycoconj J. 25(2):167-75.

Bergfeld, A.K., Claus, H., Vogel, U. and Mühlenhoff, M. (2007) Biochemical characterization of the polysialic acid specific O-acetyltransferase NeuO of Escherichia coli K1.  J. Biol. Chem. 282(30):22217-27.

Curreli, S., Arany, Z., Gerardy-Schahn, R., Mann, D., and Stamatos, N.M., (2007) Polysialylated neuropilin-2 is expressed on the surface of human dendritic cells and modulates dendritic cell-T lymphocyte interactions.  J. Biol. Chem. 282(42):30346-56.

Freiberger, F., Claus, H., Günzel, A., Oltmann-Norden, I., Vionnet, J., Mühlenhoff, M., Vogel, U., Vann, W.F., Gerardy-Schahn, R., and Stummeyer, K. (2007) Biochemical characterization of a Neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases. Mol. Microbiol. 65(5):1258-75.

Galuska, S.P., Geyer, R., Mühlenhoff, M., and Geyer, H., (2007) Characterization of Oligo- and Polysialic Acids by MALDI-TOF-MS. Anal. Chem. 79(18):7161-9.

Haile, Y., Haastert, K., Cesnulevicius, K., Stummeyer, K., Timmer, M., Berski, S., Dräger, G., Gerardy-Schahn, R., and Grothe, C. (2007) Culturing of glial and neuronal cells on polysialic acid. Biomaterials 28(6):1163-73.

Haselhorst, T., Münster-Kühnel, A.K., Oschlies, M., Tiralongo, J., Gerardy-Schahn, R., and Itzstein, M.V. (2007) Direct detection of ligand binding to Sepharose-immobilised protein using saturation transfer double difference (STDD) NMR spectroscopy. Biochem. Biophys. Res. Commun. 359(4):866-70.

Hildebrandt, H.,  Mühlenhoff, M., Weinhold, B., Gerardy-Schahn, R., (2007),  Dissecting polysialic acid and NCAM functions in brain development. J. Neurochem 103(Suppl.1): 56-64, Review

Jensen, M., Klehr, M., Vogel, A., Schmitz, S., Tawadros, S., Mühlenhoff, M., Pluck, A., Fischer, T., Schomacker, K., Schultze, J.L., and Berthold, F. (2007) One step generation of fully chimeric antibodies using Cgamma1- and Ckappa mutant mice. J. Immunother. 30(3):338-349.

Kleczka, B., Lamerz, A.C., van Zandbergen, G., Wenzel, A., Gerardy-Schahn, R., Wiese, M., and Routier, F.H. (2007) Targeted gene deletion of Leishmania major UDP-galactopyranose mutase leads to attenuated virulence. J. Biol. Chem. 282(14):10498-505.

Krylov, V., Ustyuzhanina, N., Grachev, A., Bakker, H., and Nifantiev, N. (2007) Stereoselective Synthesis of the 3-Aminopropyl Glycosides of α-D-Xyl-(1->3)-β-D-Glc and α-D-Xyl-(1->3)-α-D-Xyl-(1->3)-β-D-Glc and of Their Corresponding N-Octanoyl Derivatives. Synthesis 20:3147-3154.

Leiman, P.G., Battisti, A.J., Bowman, V.D., Stummeyer, K., Mühlenhoff, M., Gerardy-Schahn, R., Scholl, D., and Molineux, I.J. (2007) The Structures of Bacteriophages K1E and K1-5 Explain Processive Degradation of Polysaccharide Capsules and Evolution of New Host Specificities. J. Mol. Biol. 371(3):836-49.

Maggioni, A., von Itzstein, M., Gerardy-Schahn, R., and Tiralongo, J.  (2007) Targeting the expression of functional murine CMP-sialic acid transporter to the E. coli inner membrane. Biochem. Biophys. Res. Commun. 362(3):779-84.

Markram, K., Gerardy-Schahn, R., and Sandi, C. (2007) Selective learning and memory impairments in mice deficient for polysialylated NCAM in adulthood. Neuroscience 144(3):788-96.

Marx, M., Rivera-Milla, E., Stummeyer, K., Gerardy-Schahn, R., and Bastmeyer, M. (2007) Divergent evolution of the vertebrate polysialyltransferase Stx and Pst genes revealed by fish-to-mammal comparison.  Dev. Biol. 306(2):560-571.

Pekcec, A., Mühlenhoff, M., Gerardy-Schahn, R. , and Potschka, H. (2007) Impact of the PSA-NCAM system on pathophysiology in a chronic rodent model of temporal lobe epilepsy. Neurobiol. Dis. 27(1):54-66.

Rouwendal, G.J., Wuhrer, M., Florack, D.E., Koeleman, C.A., Deelder, A.M, Bakker, H., Stoopen, G.M., van Die, I., Helsper, J.P., Hokke, C.H., and Bosch, D. (2007) Efficient introduction of a bisecting GlcNAc residue in tobacco N-glycans by expression of the gene encoding human N-acetylglucosaminyltransferase III, Glycobiology. (2007) 17(3):334-44.

Schwarzer, D., Stummeyer, K., Gerardy-Schahn, R., and Mühlenhoff, M. (2007) Characterization of a novel intra-molecular chaperone domain conserved in endosialidases and other bacteriophage tail spike and fiber proteins. J. Biol. Chem. 282(5):2821-2831.

Steiner, T., Lamerz, A.C., Hess, P., Breithaupt, C., Krapp, S., Bourenkov, G., Huber, R., Gerardy-Schahn, R., and Jacob, U. (2007) Open and closed structures of the UDP-glucose pyrophosphorylase from Leishmania major. J. Biol. Chem. 282(17):13003-10.

Tiralongo, J., Fujita, A., Sato, C., Kitajima, K., Lehmann, F., Oschlies, M., Gerardy-Schahn, R., and Münster-Kühnel, A.K. (2007) The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Glycobiology 17(9):945-54.

Bakker, H., Rouwendal, G.J., Karnoup, A.S., Florack, D.E., Stoopen, G.M., Helsper, J.P., van Ree, R., van Die, I., and Bosch, D. (2006) An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes. Proc. Natl. Acad. Sci. U.S.A 103:7577-7582.

Galuska, S.P., Oltmann-Norden, I., Geyer, H., Weinhold, B., Kuchelmeister, K., Hildebrandt, H., Gerardy-Schahn, R., Geyer, R., and Mühlenhoff, M. (2006) Polysialic acid profiles of mice expressing variant allelic combinations of the polysialyltransferases ST8SiaII and ST8SiaIV. J. Biol. Chem. 281(42):31605-15.

Haselhorst, T., Stummeyer, K., Mühlenhoff, M., Schaper, W., Gerardy-Schahn, R., and von Itzstein, M. (2006) Endosialidase NF Appears To Bind PolySia DP5 in a Helical Conformation. Chembiochem. 7(12):1875-7.

Lamerz, A.C., Haselhorst, T., Bergfeld, A.K., von Itzstein, M., and Gerardy-Schahn, R. (2006) Molecular cloning of the leishmania major UDP-glucose pyrophosphorylase, functional characterisation and ligand binding analyses using NMR spectroscopy. J. Biol. Chem. 281(24):16314-22.

Seidenfaden, R., Krauter, A., Hildebrandt, H. (2006) The neural cell adhesion molecule NCAM regulates neuritogenesis by multiple mechanisms of interaction. Neurochem. Int. 49(1):1-11.

Senkov, O., Sun, M., Weinhold, B., Gerardy-Schahn, R., Schachner, M., and Dityatev, A. (2006) Polysialylated neural cell adhesion molecule is involved in induction of long-term potentiation and memory acquisition and consolidation in a fear-conditioning paradigm. J. Neurosci. 26(42): 10888-109898.

Stoenica, L., Senkov, O., Gerardy-Schahn, R., Weinhold, B., Schachner, M., Dityatev, A. (2006) In vivo synaptic plasticity in the dentate gyrus of mice deficient in the neural cell adhesion molecule NCAM or its polysialic acid. Eur. J. Neurosci. 23(9):2255-64.

Stummeyer, K., Schwarzer, D., Claus, H., Vogel, U., Gerardy-Schahn, R., Mühlenhoff, M. (2006) Evolution of bacteriophages infecting encapsulated bacteria: lessons from Escherichia coli K1-specific phages. Mol. Microbiol. 60(5):1123-35.

Tiralongo, J., Ashikov, A., Routier, F., Eckhardt, M., Bakker, H., Gerardy-Schahn, R., and von Itzstein, M. (2006) Functional expression of the CMP-sialic acid transporter in Escherichia coli and its identification as a simple mobile carrier. Glycobiology 16(1):73-81.

Ashikov, A., Routier, F., Fuhlrott, J., Helmus, Y., Wild, M., Gerardy-Schahn, R., and Bakker, H. (2005) The human solute carrier gene SLC35B4 encodes a bifunctional nucleotide sugar transporter with specificity for UDP-xylose and UDP-N-acetylglucosamine. J. Biol. Chem. 280: 27230-27235.

Bakker, H., Routier, F., Oelmann, S., Jordi, W., Lommen, A., Gerardy-Schahn, R., and Bosch, D. (2005) Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line. Glycobiology 15: 193-201.

Bakker, H., Kleczka, B., Gerardy-Schahn, R., and Routier, F.H. (2005) Identification and partial characterization of two eukaryotic UDP-galactopyranose mutases. Biol. Chem. 386: 657-661.

Bork, K., Reutter, W., Gerardy-Schahn, R., and Horstkorte, R. (2005) The intracellular concentration of sialic acid regulates the polysialylation of the neural cell adhesion molecule. FEBS Lett.  579: 5079-5083.

Fujita, A., Sato, C., Münster-Kühnel, A.K., Gerardy-Schahn, R., and Kitajima, K. (2005) Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system. Anal. Biochem. 337: 12-21.

Haselhorst, T., Münster-Kühnel, A.K., Stolz, A., Oschlies, M., Tiralongo, J., Kitajima, K., Gerardy-Schahn, R., and von Itzstein, M. (2005) Probing a CMP-Kdn synthetase by 1H, 31P, and STD NMR spectroscopy. Biochem. Biophys. Res. Commun. 327: 565-570.

Higson, A.P., Ross, A.J., Tsvetkov, Y.E., Routier, F., Sizova, O.V., Ferguson, M.A., and Nikolaev, A.V. (2005) Synthetic fragments of antigenic lipophosphoglycans from Leishmania major and Leishmania mexicana and their use for characterisation of the Leishmania elongating alpha-D-mannopyranosylphosphate transferase. Chemistry. 11: 2019-30.

Kabuss, R., Ashikov, A., Oelmann, S., Gerardy-Schahn, R., and Bakker, H. (2005) Endoplasmic reticulum retention of the large splice variant of the UDP-galactose transporter is caused by a dilysine motif. Glycobiology 15: 905-911.

Stummeyer, K., Dickmanns, A., Mühlenhoff, M., Gerardy-Schahn, R., and Ficner, R. (2005) Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F. Nat. Struct. Mol. Biol.  12: 90-96.

Weinhold, B., Seidenfaden, R., Röckle, I., Mühlenhoff, M., Schertzinger, F., Conzelmann, S., Marth, J.D., Gerardy-Schahn, R., and Hildebrandt, H. (2005) Genetic ablation of polysialic acid causes severe neurodevelopmental defects rescued by deletion of the neural cell adhesion molecule. J. Biol. Chem. 280: 42971-42977.

Horstkorte, R., Mühlenhoff, M., Reutter, W., Nohring, S., Zimmermann-Kordmann, M., and Gerardy-Schahn, R. (2004) Selective inhibition of polysialyltransferase ST8SiaII by unnatural sialic acids. Exp. Cell Res. 298: 268-274.

Kean, E.L., Münster-Kühnel, A.K., and Gerardy-Schahn, R. (2004) CMP-sialic acid synthetase of the nucleus. Biochim. Biophys. Acta 1673: 56-65.

Münster-Kühnel, A.K. , Tiralongo, J., Krapp, S., Weinhold, B., Ritz-Sedlacek, V., Jacob, U., and Gerardy-Schahn, R. (2004) Structure and function of vertebrate CMP-sialic acid synthetases. Glycobiology 14: 43R-51R.

Krapp, S., Münster-Kühnel, A.K., Kaiser, J.T., Huber, R., Tiralongo, J., Gerardy-Schahn, R., and Jacob, U. (2003) The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase. J. Mol. Biol. 334: 625-637.

Mühlenhoff, M., Stummeyer, K., Grove, M., Sauerborn, M., and Gerardy-Schahn, R. (2003) Proteolytic processing and oligomerization of bacteriophage-derived endosialidases. J. Biol. Chem. 278: 12634-12644.

Seidenfaden, R., Krauter, A., Schertzinger, F., Gerardy-Schahn, R., and Hildebrandt, H. (2003) Polysialic acid directs tumor cell growth by controlling heterophilic neural cell adhesion molecule interactions. Mol. Cell Biol. 23: 5908-5918.

Wuhrer, M., Geyer, H., von der Ohe, M., Gerardy-Schahn, R., Schachner, M., and Geyer, R. (2003) Localization of defined carbohydrate epitopes in bovine polysialylated NCAM. Biochimie 85(1-2):207-18.