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Dr. Hans Bakker

 

Ph.D.

Geb. J3, Ebene 04, Raum 1290

Tel.: +49(0)511/532-5214 oder 9806

FAX: +49(0)511/532-8801

E-Mail: bakker.hans(at)mh-hannover.de

 

 

Research interests

- Notch glycosylation

- Glycolipid specific glycosyltransferases

 

 

 DFG

 

 

Publications

      1.   Sethi,M.K., Buettner,F.F., Ashikov,A., Krylov,V.B., Takeuchi,H., Nifantiev,N.E., Haltiwanger,R.S., Gerardy-Schahn,R., and Bakker,H. (2012). Molecular Cloning of a Xylosyltransferase that Transfers the Second Xylose to O-Glucosylated Epidermal Growth Factor Repeats of Notch. J. Biol. Chem. 287, 2739-2748.

      2.   Kraft,B., Johswich,A., Kauczor,G., Scharenberg,M., Gerardy-Schahn,R., and Bakker,H. (2011). "Add-on" domains of Drosophila beta1,4-N-acetylgalactosaminyltransferase B in the stem region and its pilot protein. Cell Mol. Life Sci., 68, 4091-4100.

      3.   Sethi,M.K., Buettner,F.F., Krylov,V.B., Takeuchi,H., Nifantiev,N.E., Haltiwanger,R.S., Gerardy-Schahn,R., and Bakker,H. (2010). Identification of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats. J. Biol. Chem., 285, 1582-1586.

      4.   Bakker,H., Oka,T., Ashikov,A., Yadav,A., Berger,M., Rana,N.A., Bai,X., Jigami,Y., Haltiwanger,R.S., Esko,J.D., and Gerardy-Schahn,R. (2009). Functional UDP-xylose transport across the endoplasmic reticulum/Golgi membrane in a Chinese hamster ovary cell mutant defective in UDP-xylose Synthase. J. Biol. Chem., 284, 2576-2583.

      5.   Boztug,K., Appaswamy,G., Ashikov,A., Schaffer,A.A., Salzer,U., Diestelhorst,J., Germeshausen,M., Brandes,G., Lee-Gossler,J., Noyan,F., Gatzke,A.K., Minkov,M., Greil,J., Kratz,C., Petropoulou,T., Pellier,I., Bellanne-Chantelot,C., Rezaei,N., Monkemoller,K., Irani-Hakimeh,N., Bakker,H., Gerardy-Schahn,R., Zeidler,C., Grimbacher,B., Welte,K., and Klein,C. (2009). A syndrome with congenital neutropenia and mutations in G6PC3. N. Engl. J. Med., 360, 32-43.

      6.   Johswich,A., Kraft,B., Wuhrer,M., Berger,M., Deelder,A.M., Hokke,C.H., Gerardy-Schahn,R., and Bakker,H. (2009). Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot. J. Cell Biol., 184, 173-183.

      7.   Bakker,H., Routier,F., Ashikov,A., Neumann,D., Bosch,D., and Gerardy-Schahn,R. (2008). A CMP-sialic acid transporter cloned from Arabidopsis thaliana. Carbohydr. Res., 343, 2148-2152.

      8.   Caffaro,C.E., Luhn,K., Bakker,H., Vestweber,D., Samuelson,J., Berninsone,P., and Hirschberg,C.B. (2008). A single Caenorhabditis elegans Golgi apparatus-type transporter of UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, and UDP-N-acetylgalactosamine. Biochemistry, 47, 4337-4344.

      9.   Schmalhorst,P.S., Krappmann,S., Vervecken,W., Rohde,M., Muller,M., Braus,G.H., Contreras,R., Braun,A., Bakker,H., and Routier,F.H. (2008). Contribution of galactofuranose to the virulence of the opportunistic pathogen Aspergillus fumigatus. Eukaryot. Cell, 7, 1268-1277.

    10.   Stolz,A., Haines,N., Pich,A., Irvine,K.D., Hokke,C.H., Deelder,A.M., Gerardy-Schahn,R., Wuhrer,M., and Bakker,H. (2008). Distinct contributions of beta 4GalNAcTA and beta 4GalNAcTB to Drosophila glycosphingolipid biosynthesis. Glycoconj. J., 25, 167-175.

    11.   Kleczka,B., Lamerz,A.C., van Zandbergen,G., Wenzel,A., Gerardy-Schahn,R., Wiese,M., and Routier,F.H. (2007). Targeted gene deletion of Leishmania major UDP-galactopyranose mutase leads to attenuated virulence. J. Biol. Chem., 282, 10498-10505.

    12.   Krylov,V., Ustyuzhanina,N., Grachev,A., Bakker,H., and Nifantiev,N. (2007). Stereoselective synthesis of the 3-aminopropyl glycosides of a-D-Xyl-(1 -> 3)b-D-Glc and a-D-Xyl-(1 -> 3)-a-D-Xyl-(1 -> 3)-b-D-Glc and of their corresponding N-octanoyl derivatives. Synthesis, 2007, 3147-3154.

    13.   Rouwendal,G.J., Wuhrer,M., Florack,D.E., Koeleman,C.A., Deelder,A.M., Bakker,H., Stoopen,G.M., van,D., I, Helsper,J.P., Hokke,C.H., and Bosch,D. (2007). Efficient introduction of a bisecting GlcNAc residue in tobacco N-glycans by expression of the gene encoding human N-acetylglucosaminyltransferase III. Glycobiology, 17, 334-344.

    14.   Bakker,H., Rouwendal,G.J., Karnoup,A.S., Florack,D.E., Stoopen,G.M., Helsper,J.P., van Ree,R., van,D., I, and Bosch,D. (2006). An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes. Proc. Natl. Acad. Sci. U. S. A, 103, 7577-7582.

    15.   Tiralongo,J., Ashikov,A., Routier,F., Eckhardt,M., Bakker,H., Gerardy-Schahn,R., and von Itzstein,M. (2006). Functional expression of the CMP-sialic acid transporter in Escherichia coli and its identification as a simple mobile carrier. Glycobiology, 16, 73-81.

    16.   Ashikov,A., Routier,F., Fuhlrott,J., Helmus,Y., Wild,M., Gerardy-Schahn,R., and Bakker,H. (2005). The human solute carrier gene SLC35B4 encodes a bifunctional nucleotide sugar transporter with specificity for UDP-xylose and UDP-N-acetylglucosamine. J. Biol. Chem., 280, 27230-27235.

    17.   Bakker,H., Kleczka,B., Gerardy-Schahn,R., and Routier,F.H. (2005). Identification and partial characterization of two eukaryotic UDP-galactopyranose mutases. Biol. Chem., 386, 657-661.

    18.   Bakker,H., Routier,F., Oelmann,S., Jordi,W., Lommen,A., Gerardy-Schahn,R., and Bosch,D. (2005). Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line. Glycobiology, 15, 193-201.

    19.   Kabuss,R., Ashikov,A., Oelmann,S., Gerardy-Schahn,R., and Bakker,H. (2005). ER retention of the large splice variant of the UDP-galactose transporter is caused by a di-lysine motif. Glycobiology.

    20.   Bakker,H., Schijlen,E., de Vries,T., Schiphorst,W.E., Jordi,W., Lommen,A., Bosch,D., and van,D., I (2001). Plant members of the alpha1-->3/4-fucosyltransferase gene family encode an alpha1-->4-fucosyltransferase, potentially involved in Lewis(a) biosynthesis, and two core alpha1-->3-fucosyltransferases. FEBS Lett., 507, 307-312.

    21.   Bakker,H., Bardor,M., Molthoff,J.W., Gomord,V., Elbers,I., Stevens,L.H., Jordi,W., Lommen,A., Faye,L., Lerouge,P., and Bosch,D. (2001). Galactose-extended glycans of antibodies produced by transgenic plants. Proc. Natl. Acad. Sci. U. S. A, 98, 2899-2904.

    22.   Elbers,I.J., Stoopen,G.M., Bakker,H., Stevens,L.H., Bardor,M., Molthoff,J.W., Jordi,W.J., Bosch,D., and Lommen,A. (2001). Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves. Plant Physiol, 126, 1314-1322.

    23.   Gerardy-Schahn,R., Oelmann,S., and Bakker,H. (2001). Nucleotide sugar transporters: biological and functional aspects. Biochimie, 83, 775-782.

    24.   Stevens,L.H., Stoopen,G.M., Elbers,I.J., Molthoff,J.W., Bakker,H.A., Lommen,A., Bosch,D., and Jordi,W. (2000). Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco. Plant Physiol, 124, 173-182.

    25.   Bakker,H., Lommen,A., Jordi,W., Stiekema,W., and Bosch,D. (1999). An Arabidopsis thaliana cDNA complements the N-acetylglucosaminyltransferase I deficiency of CHO Lec1 cells. Biochem. Biophys. Res. Commun., 261, 829-832.

    26.   Sato,T., Furukawa,K., Bakker,H., Van den Eijnden,D.H., and van,D., I (1998). Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase. Proc. Natl. Acad. Sci. U. S. A, 95, 472-477.

    27.   Van den Eijnden,D.H., Neeleman,A.P., Bakker,H., and van,D., I (1998). Novel pathways in complex-type oligosaccharide synthesis. New vistas opened by studies in invertebrates. Adv. Exp. Med. Biol., 435, 3-7.

    28.   Bakker,H., Van Tetering,A., Agterberg,M., Smit,A.B., Van den Eijnden,D.H., and van Die,I. (1997). Deletion of two exons from the Lymnaea stagnalis beta1-->4-N-acetylglucosaminyltransferase gene elevates the kinetic efficiency of the encoded enzyme for both UDP-sugar donor and acceptor substrates. J. Biol. Chem., 272, 18580-18585.

    29.   Bakker,H., Schoenmakers,P.S., Koeleman,C.A., Joziasse,D.H., van Die,I., and Van den Eijnden,D.H. (1997). The substrate specificity of the snail Lymnaea stagnalis UDP-GlcNAc:GlcNAc beta-R beta 4-N-acetylglucosaminyltransferase reveals a novel variant pathway of complex-type oligosaccharide synthesis. Glycobiology, 7, 539-548.

    30.   Bakker,H., Friedmann,I., Oka,S., Kawasaki,T., Nifant'ev,N., Schachner,M., and Mantei,N. (1997). Expression cloning of a cDNA encoding a sulfotransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope. J. Biol. Chem., 272, 29942-29946.

    31.   Van den Eijnden,D.H., Bakker,H., Neeleman,A.P., Van den Nieuwenhof,I., and van Die,I. (1997). Novel pathways in complex-type oligosaccharide synthesis: new vistas opened by studies in invertebrates. Biochem. Soc. Trans., 25, 887-893.

    32.   van Die,I., Bakker,H., and Van den Eijnden,D.H. (1997). Identification of conserved amino acid motifs in members of the beta1-->4-galactosyltransferase gene family. Glycobiology, 7, v-viii.

    33.   van Die,I., Van Tetering,A., Bakker,H., Van den Eijnden,D.H., and Joziasse,D.H. (1996). Glycosylation in lepidopteran insect cells: identification of a beta 1-->4-N-acetylgalactosaminyltransferase involved in the synthesis of complex-type oligosaccharide chains. Glycobiology, 6, 157-164.

    34.   Van den Eijnden,D.H., Neeleman,A.P., Van der Knaap,W.P., Bakker,H., Agterberg,M., and van Die,I. (1995). Control and function of complex-type oligosaccharide synthesis. Novel variants of the lacNAc pathway. Adv. Exp. Med. Biol., 376, 47-52.

    35.   Van den Eijnden,D.H., Neeleman,A.P., Van der Knaap,W.P., Bakker,H., Agterberg,M., and van Die,I. (1995). Novel glycosylation routes for glycoproteins: the lacdiNAc pathway. Biochem. Soc. Trans., 23, 175-179.

    36.   Bakker,H., Agterberg,M., Van Tetering,A., Koeleman,C.A., Van den Eijnden,D.H., and van Die,I. (1994). A Lymnaea stagnalis gene, with sequence similarity to that of mammalian beta 1-->4-galactosyltransferases, encodes a novel UDP-GlcNAc:GlcNAc beta-R beta 1-->4-N-acetylglucosaminyltransferase. J. Biol. Chem., 269, 30326-30333.

    37.   de Boer,D., Bakker,H., Lever,A., Bouma,T., Salentijn,E., and Weisbeek,P. (1991). Protein targeting towards the thylakoid lumen of chloroplasts: proper localization of fusion proteins is only observed in vivo. EMBO J., 10, 2765-2772.

    38.   Fillat,M.F., Bakker,H.A., and Weisbeek,P.J. (1990). Sequence of the ferredoxin-NADP(+)-reductase gene from Anabaena PCC 7119. Nucleic Acids Res., 18, 7161.