Dietmar J. Manstein
PhD 1986 Heidelberg, 1987 - 1990 Postdoctoral work at Stanford University School of Medicine, USA.
1990 - 1996 Group Leader at the National Institute for Medical Research, London, UK.
1996 - 2002 Senior Scientist at the Max-Planck-Institute for Medical Research, Heidelberg.
Since 2002 Director of the Institute for Biophysical Chemistry (OE 4350) and the Division for Structural Analysis (OE 8830) at Hannover Medical School.
Motor protein-linked diseases; structure-function relationships in molecular motors; regulation of actin-based contractility; ATPases, GTPases, C-C-bond-cleaving oxidoreductases.
X-ray crystallography; drug-design; time-resolved spectroscopy; transient kinetics; live-cell microscopy
The objective of my work is the characterization of molecular motors and proteins that regulate dynamic changes of cytoskeletal and membranous structures. The coordinated generation of movement and force is essential for basic processes such as cell division, phagocytosis, chromosome segregation, muscle contraction, and axonal transport.
Elucidation of the molecular mechanisms underlying motile events is of significance with respect to a wide range of health related issues, such as neurodegeneration, heart failure, skeletal muscle myopathies, cell-mediated immune response, wound healing, and the invasion of healthy tissue by malignant tumor cells.
Our experiments address the role of isoform-specific differences, disease-causing mutations, and drugs by integrating information derived from examining contractile events at several levels of organization. At the single molecule level, the work examines the basic design and function of the molecular motors, actin filaments, and regulatory proteins using highly-sensitive and fast techniques to follow chemical, spectroscopic, and mechanical changes. These studies are usually combined with protein engineering and high-resolution structural analyses. At the level of isolated cells, the research program addresses the specific roles of human myosin, actin and tropomyosin isoform for the assembly of the contractile apparatus.
Prof. Dr. Dietmar J. Manstein
Institute for Biophysical Chemistry, OE4350
Medizinische Hochschule Hannover